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Yeasen Biotechnology superscript iii reverse transcriptase
Superscript Iii Reverse Transcriptase, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+reverse+transcriptase/reverse+transcriptase/pmc13191876-60-21-34
Average 86 stars, based on 1 article reviews
superscript iii reverse transcriptase - by Bioz Stars, 2026-11
86/100 stars

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Extraction:

Article Title: Transcription factor c-Maf drives macrophages to promote hypertrophic scar formation.
Article Snippet: Following a co- culture for 8 h, the NFBs or HFBs were fixed with methanol for 25 min, washed with PBS for three times, and stained with 0.5% crystal violet solution for 30 min. Then, the cells on the upper side of membrane were removed, cells on the lower side of membrane were examined and photographed using a microscope (Olympus, FSX100). .. The extraction of total RNA was the same with our previous descriptions of Lin Chen et al.11 Superscript II® (yeasen, Superscript®Reverse Transcriptase) was used for the production of cDNA. .. The Real- time PCR System (BIO- RAD, CFX Connect, US) was employed to perform quantitative polymerase chain reaction (qPCR) using Platinum SYBR Green (Yeasen, 11184ES08).

Article Title: Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways.
Article Snippet: A CLINX + system (CLINX, China) was exploited to examine the bound immunocomplexes. .. Following extraction of total RNA from cells by adoptingTRIzol reagent (1 mL; Invitrogen, USA), 1 μg of the total RNA extract was transcribed reversely as per the protocol of Superscript II reverse transcriptase (Yeasen, Shanghai, China) using oligo (T) primers. .. SYBR Green PCR Master Mix (Yeasen, Shanghai, China) was utilized to quantify the expression of gene on an ABI7300.

Cell Culture:

Article Title: Clinical and Molecular Landscape of GLRA2 in X-Linked Early-Onset High Myopia.
Article Snippet: Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. .. Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. ..

Article Title: Pathogenic relationship between phenotypes of ARPKD and novel compound heterozygous mutations of PKHD1
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers ( ). ..

Reverse Transcription:

Article Title: Clinical and Molecular Landscape of GLRA2 in X-Linked Early-Onset High Myopia.
Article Snippet: Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. .. Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. ..

Article Title: Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways.
Article Snippet: A CLINX + system (CLINX, China) was exploited to examine the bound immunocomplexes. .. Following extraction of total RNA from cells by adoptingTRIzol reagent (1 mL; Invitrogen, USA), 1 μg of the total RNA extract was transcribed reversely as per the protocol of Superscript II reverse transcriptase (Yeasen, Shanghai, China) using oligo (T) primers. .. SYBR Green PCR Master Mix (Yeasen, Shanghai, China) was utilized to quantify the expression of gene on an ABI7300.

Article Title: Pathogenic relationship between phenotypes of ARPKD and novel compound heterozygous mutations of PKHD1
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers ( ). ..

Article Title: Design and Construction of Carboxylesterase 2c Gene Knockout Rats by CRISPR/Cas9.
Article Snippet: Background: Carboxylesterase 2 (CES2) is mainly distributed in the human liver and gut, and plays an active role in the metabolic activation of many prodrugs and lipid metabolism.. Although CES2 is of great significance, there are still few animal models related to CES2.. Objectives: This research aims to construct Ces2c gene knockout (KO) rats and further study the function of CES2.

cDNA Synthesis:

Article Title: Clinical and Molecular Landscape of GLRA2 in X-Linked Early-Onset High Myopia.
Article Snippet: Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. .. Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. ..

Article Title: Pathogenic relationship between phenotypes of ARPKD and novel compound heterozygous mutations of PKHD1
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers ( ). ..

Real-time Polymerase Chain Reaction:

Article Title: Clinical and Molecular Landscape of GLRA2 in X-Linked Early-Onset High Myopia.
Article Snippet: Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. .. Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. ..

Article Title: Pathogenic relationship between phenotypes of ARPKD and novel compound heterozygous mutations of PKHD1
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers ( ). ..

Polymerase Chain Reaction:

Article Title: Clinical and Molecular Landscape of GLRA2 in X-Linked Early-Onset High Myopia.
Article Snippet: Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. .. Total RNA was extracted from cells cultured for 48 hours with Trizol (RNAiso PLUS; TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR; YEASEN, Shanghai, China), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers. ..

Article Title: Pathogenic relationship between phenotypes of ARPKD and novel compound heterozygous mutations of PKHD1
Article Snippet: .. Total RNA was extracted from cells cultured for 48 h with Trizol (RNAiso PLUS) (TaKaRa, Kusatsu, Japan) and reverse-transcribed with the Superscript III reverse transcriptase (HifairTM 1st Strand cDNA Synthesis SuperMix for qPCR (YEASEN, Shanghai, China)), and the resulting cDNA was PCR-amplified with pcMINI-F/pcMINI-R primers ( ). ..

Recombinase Polymerase Amplification:

Article Title: Establishment of the recombinase polymerase amplification–lateral flow dipstick (RPA–LFD) detection technique for rapid detection of lymphocystis disease virus (LCDV)
Article Snippet: Lymphocystis disease (LCD), induced by the lymphocystis disease virus (LCDV), results in losses across more than 140 fish species.. In this study, we present a rapid, convenient, and highly specific detection assay based on recombinase polymerase amplification–lateral flow dipstick (RPA–LFD) technology to identify LCDV.. This assay targets the highly conserved major capsid protein (MCP) of LCDV using specific and sensitive primers and probes.

other:

Article Title: O -GlcNAcylation Coordinates Glutaminolysis by Regulating the Stability and Membrane Trafficking of ASCT2 in Hepatic Stellate Cells
Article Snippet: Total RNA was extracted from cells using Trizol (Invitrogen).



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